Peculiarities of immunopathogenesis of interstitial cystitis/syndrome of a pained bladder


The aim of the study was to determine the expression of cytokines, mast cells quantity in the bladder mucosa and their relationship in patients with IC/BPS.

Material and methods. A total of 126 women with clinically diagnosed IC/BPS were examined, mean age 46.7 ± 14.0 years. The concentration of interleukins (IL)-1β, IL-6, IL-8, tumor necrosis factor a (TNFα) in blood serum was determined by ELISA. Mast cells were identified in biopsy specimens of the bladder mucosa taken during cystoscopy using histological analysis.

Statistical analysis was performed using the Statistica program in Microsoft Excel release 6 (StatSoft, USA). The Pearson correlation coefficient is calculated.

Result. In the main group, moderate pain was observed in 56.3 % of cases, and urination more than 8 times a day in 59.5 % of cases. The average level of pro-inflammatory cytokines -IL-1β, IL-6, IL-8 and TNFα in the blood serum of the examined main group was higher than in the comparison group by 63.34, 66.67, 68.83 and 70.16 % respectively. The number of mast cells averaged 82.27 ± 38.76. Mast cells number correlated with IL-6 and IL-8 weak, with TNFa - a noticeable relationship (r = -0,418). There was a moderate negative correlation between the mast cells number and the level of TNFα (r = -0.418), no significant correlation with the levels of IL-6 and IL-8 was found.

Conclusion. The identification of cytokines in patients with IC/BPS can be used to improve the diagnosis and exclusion criteria for this pathology. Analysis of serum cytokines and mast cells is a convenient approach to monitoring the activation of inflammatory cells in the tissue of the bladder.

Keywords:interstitial cystitis/bladder pain syndrome; cytokines; mast cells; biopsy; correlation

For citation: Sholan R.F. Peculiarities of immunopathogenesis of interstitial cystitis/syndrome of a pained bladder. Immunologiya. 2019; 41 (3): 274-9. DOI: 10.33029/0206-4952-2020-41-3-274-279 (in Russian)

Funding. The study had no sponsor support.

Conflict of interests. The author declare no conflict of interests.


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Rakhim M. Khaitov
Аcademician of the Russian Academy of Sciences, MD, Professor, Honoured Science Worker of the Russian Federation, Scientific Director of the NRC Institute of Immunology FMBA of Russia, Chief Allergist-Immunologist of the Ministry of Health of the Russian Federation

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