Morphological characteristics of white pulp and spleen dendritic cells at the experimental carcinogenesis

• Arlashkina O.M.
• Struchko G.Yu.
• Merkulova L.M.
• Mikhaylova M.N.

Abstract

By means of histological methods the morphology and an immunohistochemistry of structures of a lien after course introduction 1,2-dimethylhydrazines is studied. It is established that in 1 month after administration of carcinogen in a lien of rats the augmentation of quantity of S100⁺-cells in the germinative the centers of lymphoid nodules against the background of reduction of both the area of nodules, and the centers of reproduction, width of marginal zones and periarterial lymphoid couplings is registered. Besides, the total of secondary lymphoid nodules against the background of decrease of their ratio to primary lymphoid nodules is enlarged. In 4 months after the termination of a course of injections of carcinogen doubtful decrease of quantity of dendritic cells in the germinative the centers in comparison with intact animals of the same age period becomes perceptible. Changes of a morphological phenotype of a lien on this term have reliable character and width of periarterial lymphoid couplings and marginal zones, and also augmentation of quantity of secondary lymphoid nodules consist in more expressed reduction of the areas of lymphoid nodules with their germinative centers.

Keywords:lien; carcinogenesis; 1,2-dimethylhydrazine; whitepulp; dendriticcells

References

1. Mebius R.E., Kraal G. Structure and function of the spleen. Nat. Pev. Immunal. 2005; 5: 606-16.

2. Shapkin Yu.G., Maslyakov V.V. Lien and immune status of an organism. Vestnik khirurgii. 2009; 168 (2): 110-3. (in Russian)

3. Balogh P., Horvath G., Szakal A.K. Immunoarhitecture of distinct reticular fi broblastic domains in the white pulp of mouse spleen. J. Histochem. Cytochem. 2004; 52 (1287): 98.

4. Khaitov R.M. Physiology of the immune system. Rossiyskiy fiziologicheskiy zhurnal im. I.M. Sechenova. 2000; 86 (3): 252-6. (in Russian)

5. Vorob’ev A.A., Bykov A.S., Karaulov A.V. Immunology and allergology: a color atlas. Moscow: Prakticheskaya meditsina, 2006: 64-74. (in Russian)

6. Shibazaki M., Nakamura M., Nitta Y., Endo Y. Immunopharmacology. 1998; 39 (1): 1-7.

7. Mailloux A.W., Young M.R. Regulatory T-cell trafficking: from thymic development to tumor-induced immune suppression. Crit. Rev. Immunol. 2010; 30 (5): 435-47.

8. Mikhaylova M.N., Struchko G.Yu., Merkulova L.M., Kostrova O.Yu., et al. The involvement of dendritic and neuroendocrine cells of the thymus in the development of its involution in the formation of an experimental colon tumor. Vestnik Chuvashskogo universiteta. 2011; 3: 377-83. (in Russian)

9. Volkov V.P. A new algorithm for the morphometric evaluation of the functional immunomorphology of the spleen. Universum: Meditsina i farmakologiya: elektronniy nauchniy zhurnal. 2015; 5-6 (18) [Electronic Resource]. URL: http://7universum.com/ru/med/ archive/item/2341. (date of access April 10, 2017). (in Russian)

10. Rebrova O.Yu. Statistical analysis of medical data. Application of the STATISTICA software package. Moscow: Media Sfera, 2002. (in Russian)

11. Struchko G.Yu., Merkulova L.M., Moskvichev E.V., Kostrova O.Yu., et al. The morphological and immunohistochemical characteristic of tumors of digestive tract against the background of an immune failure. Vestnik Chuvashskogo universiteta. 2011; 3: 450-6. (in Russian)

12. Wood G.S., Turner N.R., Shiurba R.A., Eng L., et al. Human dendritic cells and macrophages: ill sitll immunophenotypic definition of subsets that exhibit specific morphologic and microenv ironmenta l characteristics. Am. J. Pathol. 1985; 119: 73-82.

13. Alos L., Navarrete P., Morente V., et al. Mod. Pathol. 2005; 18 (1): 127-36.

14. Moskvichev E.V., Merkulova L.M., Struchko G.Yu., Mikhaylova M.N., et al. The immunohistochemical characteristic of an aktsidentalny involution of a thymus after a splenectomy. Vestnik Natsional’nogo mediko-khirurgicheskogo tsentra N.I. Pirogova. 2012; 7 (2): 40-3. (in Russian)

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