Analysis of IL1B (rs1143627) IL4 (rs2243250), IL6 (rs1800795), IL8 (rs4073), IL10 (rs1800896, rs1800872), IL17A (rs227593) cytokines genes polymorphism and its complex genotypes among Caucasian patients of Western Siberia with primary open-angle glaucoma

Abstract

Introduction. The significance of the relationship between cytokine genes polymorphism and the development of various forms of glaucoma has recently become one of the significant research topics.

Aim of the study - the analysis of promoter polymorphism of cytokine genes IL1B (rs1143627), IL4 (rs2243250), IL6 (rs1800795), IL8 (rs4073), IL10 (rs1800896), IL10 (rs1800872), IL17A (rs227593) and its complex in a group of patients with primary open-angle glaucoma (POAG) relatively healthy individuals to identify the association with the disease.

Material and methods. 99 patients with stage II POAG were examined. The comparison group included 100 people. Single-nucleotide polymorphism was studied by Real-Time PCR with SYBR Green I. Significance of differences in alternative groups was determined using 2-sided Fisher’s exact test with Bonferroni correction.

Results. IL1B-31*CC minor genotype, IL8-251*TT wild type genotype and IL8-251* TT:IL17-197*AA complex genotype are decrease in patients. Frequency distribution differences of positively and negatively associated genotypes with pathology in groups of men and women were revealed.

Conclusion. The analysis made it possible to identify the features of the frequency distribution of the genotypes of the studied genes associated with the development of the disease.

Keywords:primary open-angle glaucoma; cytokines genes; complex genotypes; single nucleotide polymorphism

For citation: Shevchenko A.V., Prokof’ev V.F., Konenkov VI., Chernykh V.V, Ermakova O.V, Trunov A.N. Analysis of IL1B (rs1143627), IL4 (rs2243250), IL6 (rs1800795), IL8 (rs4073), IL10 (rs1800896, rs1800872), IL17A (rs227593) cytokines genes polymorphism and its complex genotypes among Caucasian patients of Western Siberia with primary open-angle glaucoma. Immunologiya. 2021; 42 (3): 211-21. DOI: https://doi.org/10.33029/0206-4952-2021-42-3-211-221 (in Russian)

Funding. The study had no sponsor support.

Conflict of interests. The authors declare no conflict of interests.

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