4 . 2021

Local cytokine status in patients with sialadenosis of parotid glands with hypothyroidism

Abstract

Introduction. The salivary glands’ dystrophic and inflammatory diseases occupy a significant proportion of the prevalence of dental pathology. It is known that the pathogenesis of sialadenosis is associated with thyroid gland dysfunction. Local treatment of sialadenosis and correction of somatic disease is not enough, as it’s not possible to achieve in these patients stable remission. It has been experimentally proved that already in subclinical forms of hypothyroidism there are morphological changes in the parenchyma of parotid gland with lymphoid infiltration. The study of local cytokine levels can largely determine outcome of the disease.

Aim - to study pro- and anti-inflammatory cytokine levels in parotid secretion of patients with sialadenosis and hypothyroidism.

Material and methods. The study consisted of 52 patients with sialadenosis of parotid glands. All the patients were divided into 2 groups: 1^st - 30 patients in remission stage, 2^nd -22 patients in the acute stage. The comparison group were included 20 healthy volunteers. The content of IL-1β, IL-6, TNFα and IL-10 was determined in the parotid secretion by enzyme immunoassay (ELISA) using Vector-Best reagents (Russia) and BMG Labtech (ClarioStar, Germany).

Results. There was a significant difference in the levels of IL-1β, IL-6, TNF-α between the groups of sialadenosis patients and the group of comparison with the maximum cytokines’ level under study in sialodenosis in the acute stage. The level of IL-10 was significantly lower in sialodenosis in the acute stage compared to the other groups. TNFα was detected in parotid secretion only in 25 % of subjects in the group of comparison.

Conclusion. The study of proinflammatory and anti-inflammatory cytokines in the salivary gland secretion, along with the clinical picture, can serve as an additional diagnostic tool to determine the severity of the inflammatory process in these patients, which allows to consider the use of this method in practical dentistry for dynamic monitoring of patients and further application of treatment efficacy.

Keywords:salivary glands; sialadenosis; saliva; cytokines; interleukins; TNFα; hypothyroidism; parotid secretion; xerostomia; hyposalivation

For citation: Sultimova T.B., Kozlova M.V., Gaponov A.M., Savlevich E.L., Kozlov I.G. Local cytokine status in patients with sialadenosis of parotid glands with hypothyroidism. Immunologiya. 2021; 42 (4): 356-63. DOI: https://doi.org/10.33029/0206-4952-2021-42-4-356-363 (in Russian)

Funding. The study had no sponsor support.

Conflict of interests. The authors declare no conflict of interests.

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