Complex assessment of the cytokine profile, proteinograms and inflammation acute phase proteins in patients with acute brucellosis
AbstractIntroduction. The pathogenesis of brucellosis is directly related to the induction of proinflammatory cytokines and activation of the Th1-type immune response. The role of inflammatory factors in acute brucellosis remains unexplored.
Aim of the study - to determine the level of proinflammatory cytokines (IL-1β, IL-6, IL-8, IL-12, IL-18 and IFN-γ), proteins of the acute phase of inflammation (neopterin and lipopoly-saccharide-binding protein) and features of the fractional composition of blood serum proteins in patients with acute brucellosis before and after complex antibiotic therapy and taking an immunomodulator Licopid®.
Material and methods. The object of the study were clinical blood samples from 65 patients with a laboratory-confirmed diagnosis of acute brucellosis, admitted to the State Medical Institution «City Clinical Hospital No. 2», Stavropol. The concentrations of cytokines (IL-1β, IL-8, IL-12, IL-18, IFN-γ) and acute phase proteins were determined in blood serum by enzyme-linked immunosorbent assay, IL-6 level - by immunochemiluminescence method, fractional composition of blood serum proteins - using electrophoresis.
Results. In the acute phase of brucellosis infection (before treatment), there is a high level of pro-inflammatory cytokines IL-1β, IL-6, IL-8, IL-18 and IFN-γ. After the course of antibiotic therapy (doxycycline and rifampicin, 6 weeks), high level of IL-8 and IL-18 remains in the blood serum of patients with moderate brucellosis, indicating active inflammation. The level of LPS protein and neopterin decreased statistically significant during therapy. Dysproteinemia was shown with an increase in the level of globulins, mainly due to α- and γ-globulin fractions, a moderate decrease in the concentration of total protein, hypoalbuminemia, and a decrease in the albumin-globulin coefficient.
Conclusion. The prospect of further study of the cytokine profile (IL-1β, IL-6, IL-8, IL-12, IL-18, INF-γ) to assess the intensity of the systemic response to infection and to predict the course of acute brucellosis is noted.
Keywords:brucellosis; proteinogram; cytokines; acute phase proteins; Licopid®
For citation: Sarkisyan N.S., Kulichenko A.N., Kovalevich N.I., Sannikova I.V., Makhinya O.V. Complex assessment of the cytokine profile, proteinograms and inflammation acute phase proteins in patients with acute brucellosis. Immunologiya. 2021; 42 (5): 511-7. DOI: https://doi.org/10.33029/0206-4952-2021-42-5-511-517 (in Russian)
Funding. The study had no sponsor support.
Conflict of interests. The authors declare no conflict of interests.
References
1. Boschiroli M.L., Foulongne V., Callaghan D.O. Brucellosis: a worldwide zoonosis. Curr. Opin. Microbiol. 2001; 4: 58–64. DOI: https://doi.org/10.1016/s1369-5274(00)00165-x
2. Liautard J. P., Gross A., Dornand J. Interactions between professional phagocytes and Brucella spp. Microbiologia. 1996; 12 (2): 197–206. URL: https://www.semanticscholar.org/paper/Interactions-between-professional-phagocytes-and-Liautard-Gross/d049bb02c9ded49df92ba190bba250b63f55ae35
3. Zhumadilova Z. K. Baysugurov Zh. B. Enzyme systems of phagocytic mono-nuclears in various variants of the course of brucellosis. Healthcare of Kazakhstan. 1989; 6: 29–31. (in Russian).
4. Mantur B.G., Amarnath S.K., Shinde R.S. Review of clinical and laboratory features of human brucellosis. J. Indian Med. Microbiol. 2007; 25 (3): 188–202. DOI: https://doi.org/10.4103/0255-0857.34758
5. Vasil’eva E.V., Lapin S.V., Blinova T.V. Comparative value of the quantiferon test, neopterin and specific anti-tuberculosis antibodies for clinical laboratory diagnosis of pulmonary tuberculosis. Clinical laboratory diagnostics. 2013; 5: 21–6. URL: https://cyberleninka.ru/article/n/sravnitelnaya-tsennost-kvantiferonovogo-testa-neopterina-i-spetsificheskih-protivotuberkuleznyh-antitel-dlya-kliniko-laboratornoy (in Russian)
6. Sannikova I.V., Makhinya O.V., Maleev V.V., Deyneka D.A., Golub O.G., Koval’chuk I.V., Lyamkin G.I. Brucellosis in the Stavropol Territory: results of 15-year observation of epidemiological and clinical features. Therapeutic Archive. 2015; 78 (11): 11–7. DOI: https://doi.org/10.17116/terarkh2015871111-17 (in Russian)
7. Ariza J., Corredoira J., Pallares R., Viladrich P. F., Rufi G., Pujol M., Gudiol F. Characteristics of and risk factors for relapse of brucellosis in humans. Clin. Infect. Dis. 1995; 20: 1241–9. DOI: https://doi.org/10.1093/clinids/20.5.1241
8. Gladkikh R.A., Molochny V.P., Polesco I. V. Neopterin as a modern marker of inflammation. Children's infections. 2016; 5 (2): 19–23. DOI: https://doi.org/10.22627/2072-8107-2016-15-2-19-23 (in Russian)
9. Smirnov I.E., Mityushin I.L., Kucherenko A.G., Bakradze M. D. Cytokine profile in bacterial and viral infection. Russian Pediatric Journal. 2014; 4: 14–9. URL: http://cyberleninka.ru/article/n/tsitokinovyy-profil-pri-bakterialnoy-i-virusnoy-infektsii-u-detey (in Russian)
10. Skendros P., Pappas G., Boura P. Cell-mediated immunity in human brucellosis. J Microbes Infect. 2011; 13 (2): 134–42. DOI: https://doi.org/10.1016/j.micinf.2010.10.015
11. Sviridov E. A., Telegina T. A. Neopterin and its reduced forms: biological role and participation in cellular immunity. Advances in biological chemistry. 2005; 45: 355–90. URL: https://www.fbras.ru/wp-content/uploads/2017/10/sviridov (in Russian)
12. Lyapina E.P., Shul’dyakov A.A., Spirina V.F. Chronic brucellosis: etiology, pathogenesis, clinic, treatment. Saratov: Tactik-Studio, 2011: 160 p. ISBN: 978-5-916-032-4. (in Russian)
13. Yushchuk N.D., Vengerov Yu.Ya. Communicable diseases: national guidelines. Moscow: GEOTAR-Media, 2019: 848 p. ISBN: 978-5-9704-4817-5. (in Russian)
14. Lyamkin G.I., Ponomarenko D.G., Khudoleev A.A., Rusanova D.V., Vilinskaya S.V., Kulichenko A.N. Review of the epidemiological situation of brucellosis in the Russian Federation in 2015 and forecast for 2016. Problems of particularly dangerous infections. 2016; (2): 11–3. DOI: https://doi.org/10.21055/0370-1069-2016-2-11-13 (in Russian)