2 . 2022

Peripheral blood B-lymphocyte subpopulations coexpressing CD5 and chemokine receptors in patients with chronic obstructive pulmonary disease

Abstract

Introduction. Autoantibodies to the lung cells, cell components, and extracellular matrix proteins were found in the lung tissue of patients with chronic obstructive pulmonary disease (COPD). CD5⁺ B-lymphocytes may be a significant source of these autoantibodies. However, the quantitative changes and the ability to migrate to the site of inflammation of this subpopulation of lymphocytes in patients with COPD remain unclear.

The aim of the study was to determine the quantitative changes in the subpopulations of B-lymphocytes expressing CD5 and chemokine receptors in the peripheral blood of patients with COPD.

Material and methods. The current study involved 51 smoking patients with COPD, 21 healthy smokers and 20 healthy non-smokers. Coexpression of CD5 and chemokine receptors CXCR3, CXCR4, CXCR5, and CCR7 in the peripheral blood was assessed using flow cytometry.

Results. There was a significant increase in the percentage of blood СD5⁺ B-cells, as well as B-lymphocytes coexpressing CD5 and CXCR3 in smoking patients with COPD compared with healthy smokers and healthy non-smokers. The proportion of peripheral blood B-cells expressing chemokine receptor CXCR3 but lacking CD5 was also higher in COPD patients than in healthy smokers and healthy non-smokers. The percentage of CD5⁺CXCR3^high B-cells was increased in COPD patients with GOLD stages 3–4 compared with patients having GOLD stage 2. CAT score that denotes quality of life of COPD patients was positively correlated with the proportion of blood CD5⁺CXCR3^high and CD5^-CXCR3^high B-lymphocytes. There were no differences in the proportion of CD5⁺ and CD5^– B-lymphocytes expressing chemokine receptors CXCR4, CXCR5 and CCR7 between patients with COPD and healthy subjects. In all the three subject groups, a higher percentage of CD5⁺ B-cells expressing CXCR3 and CXCR4 and a lower percentage of CD5⁺ B-cells expressing CXCR5 was observed compared with the proportion of CD5^– B-lymphocytes positive for corresponding receptors.

Conclusion. The results of the study demonstrate changes in the profile of B-lymphocyte chemokine receptors in peripheral blood of COPD patients depending on CD5 coexpression.

Keywords:B-lymphocytes; chemokine receptors; chronic obstructive pulmonary disease; CD5; CXCR3; CXCR4; CXCR5; CCR7

For citation: KadushkinA.G., TahanovichA.D., MovchanL.V., ZafranskayaM.M., DziadzichkinaV.V., Shman T.V. Peripheral blood B-lymphocyte subpopulations coexpressing CD5 and chemokine receptors in patients with chronic obstructive pulmonary disease. Immunologiya. 2022; 43 (2): 197–207. DOI: https://doi.org/10.33029/0206-4952-2022-43-2-197-207 (in Russian)

Funding. The study was supported by the Belarusian State Scientific Program «Fundamental and Applied Sciences for Medicine» (grant number 2.18).

Conflict of interests. The authors declare no conflict of interests.

Authors’ contribution. The concept and design of the study – Kadushkin A.G., Tahanovich A.D., Shman T.V.; conducting experiments – Kadushkin A.G., Movchan L.V., Zafranskaya M.M.; analysis and statistical processing of data – Kadushkin A.G., DziadzichkinaV.V.; text writing, editing – Kadushkin A.G., Tahanovich A.D., Shman T.V.

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