Characteristics of monocyte differentiation and CD163 expression in women with pointed early mission

Abstract

Introduction. Despite the advances in diagnosis and treatment, reproductive losses before 22 weeks of gestation remain one of the priority problems of modern medicine.

The aim – to assess the character of monocyte differentiation and the level of cells expressing the scavenger receptor CD163 in threatened early miscarriage in dependency of pregnancy outcome.

Material and methods. 115 women at 5–12 weeks of gestation were included into the investigation 80 of them with threatened early miscarriage (main group) and 35 women without signs of threatened miscarriage (comparison group). The relative content of classically activated, intermediate, alternatively activated monocytes expressing the scavenger receptor CD163 in the monocyte gate was assessed using FACScanto II flow cytometer (Becton Dickinson, USA).

Results. In women of the main group, the amount of classically activated and intermediate monocytes did not differ from the parameters of the comparison group and didn’t change depending on the outcome of pregnancy. However, the level of alternatively activated monocytes in women of the main group, whose pregnancy ended with reproductive losses up to 22 weeks of pregnancy, was lower than in the comparison group and in women of the main group, whose pregnancy ended in timely delivery. In women of the main group, the content of classically activated and intermediate monocytes, expressing CD163, did not differ from those in the comparison group and did not depend on the outcome of pregnancy. However, the content of alternatively activated monocytes expressing CD163, in women of the main group, whose pregnancy ended in reproductive losses before 22 weeks of gestation, was higher both in comparison with the parameters of comparison group and those of women of the main group, whose pregnancy ended in timely delivery.

Conclusion. Population of alternatively, activated monocytes, expressing scavenger receptor CD163, might participate in the pathogenesis of threatened early miscarriage, and their peripheral blood levels can be used as the predictor of reproductive losses up to 22 weeks in women with threatened early miscarriage.

Keywords:pregnancy; threatened early miscarriage; reproductive losses; monocytes; scavenger receptor CD163

For citation: Sotnikova N.Yu., Farzalieva A.V., Borzova N.Yu., Voronin D.N., Kroshkina N.V. Characteristics of monocyte differentiation and CD163 expression in women with pointed early mission. Immunologiya. 2022; 43 (6): 714–21. DOI: https://doi.org/10.33029/0206-4952-2022-42-6-714-721 (in Russian)

Funding. The study had no sponsor support.

Conflict of interests. The authors declare no conflict of interests.

Authors’ contribution. The concept and design of the study – Sotnikova N.Yu., Borzova N.Yu.; collection and processing of material – Farzalieva A.V., Kroshkina N.V.; statistical processing – Farzalieva A.V., Voronin D.N.; writing the text – Sotnikova N.Yu., Farzalieva A.V.; editing – Sotnikova N.Yu., Farzalieva A.V., Voronin D.N.; approval of the final version of the article – Sotnikova N.Yu., Farzalieva A.V., Borzova N.Yu., Voronin D.N.; responsibility for the integrity of all parts of the article – Sotnikova N.Yu., Farzalieva A.V., Borzova N.Yu., Voronin D.N.

References

1. Savely’eva G.M., Sukhikh G.T., Serova V.N., Radzinsky V.E. Obstetrics: A national guide. 2nd ed., rev. and add. Moscow: GEOTAR-Media, 2015: 1080 p. (in Russian)

2. Podzolkova N.M., Skvortsova M.Yu., Denisova T.V. Spontaneous termination of pregnancy: modern approaches to diagnosis, treatment and prevention. Moscow: GEOTAR-Media; 2018: 224 с. (in Russian)

3. Khamatova A.A., Chebotareva T.A., Balmasova I.P. Tissue-resident natural killer cells: features of functioning in the uterus and decidual membrane. Immunologiya. 2021; 42 (5): 574–80. DOI: https://doi.org/10.33029/0206-4952-2021-42-5-574-580 (in Russian)

4. Watanabe R., Hashimoto M. Pathogenic role of monocytes/macrophages in large vessel vasculitis. Front. Immunol. 2022; 13: 859502. DOI: https://doi.org/10.3389/fimmu.2022.859502 PMID: 35967455; PMCID: PMC9372263.

5. Ataullakhanov R.I., Ushakova E.I., Al’ Khudhur S.А., Pichugin A.V., Lebedeva E.S. Reprogramming of myeloid cells of the tumor microenvironment – a new approach in the immunotherapy of malignant neoplasms. Immunologiya. 2022; 43 (4): 375–88. DOI: https://doi.org/10.33029/0206-4952-2022-43-4-375-388 (in Russian)

6. Ziegler-Heitbrock L., Hofer T.P. Toward a refined definition of monocyte subsets. Front. Immunol. 2013; 4: 23. DOI: https://doi.org/10.3389/fimmu.2013.00023 PMID: 23382732; PMCID: PMC3562996.

7. Maligianni I., Yapijakis C., Nousia K., Bacopoulou F., Chrousos G.P. Exosomes and exosomal non-coding RNAs throughout human gestation (Review). Exp. Ther. Med. 2022; 24 (3): 582. DOI: https://doi.org/10.3892/etm.2022.11518 PMID: 35949320; PMCID: PMC9353550.

8. Bai K., Li X., Zhong J., Ng E.H.Y., Yeung W.S.B., Lee C.L., Chiu P.C.N. Placenta-derived exosomes as a modulator in maternal immune tolerance during pregnancy. Front. Immunol. 2021; 12: 671093. DOI: https://doi.org/10.3389/fimmu.2021.671093 PMID: 34046039; PMCID: PMC8144714.

9. Sun Y., Wu S., Zhou Q., Li X. Trophoblast-derived interleukin 9 mediates immune cell conversion and contributes to maternal-fetal tolerance. J. Reprod. Immunol. 2021; 148: 103379. DOI: https://doi.org/10.1016/j.jri.2021.103379

10. Yeganeh Kazemi N., Fedyshyn B., Sutor S., Fedyshyn Y., Markovic S., Enninga E.A.L. Maternal monocytes respond to cell-free fetal DNA and initiate key processes of human parturition. J. Immunol. 2021; 207: 2433–44. DOI: https://doi.org/10.4049/jimmunol.2100649 PMID: 34663619; PMCID: PMC8578468.

11. Faas M.M., Spaans F., De Vos P. Monocytes and macrophages in pregnancy and pre-eclampsia. Front. Immunol. 2014; 298 (5). DOI: https://doi.org/10.3389/fimmu.2014.00298 PMID: 25071761; PMCID: PMC4074993.

12. Bai K., Lee C.L., Liu X., Li J., Cao D., Li Z., Hu D., Li H., Hou Y., Xu Y.,. Kan A.S.Y., Cheung K.W., Ng E.H.Y., Yeung W.S.B., Chiu P.C.N. Human placental exosomes induce maternal systemic immune tolerance by reprogramming circulating monocytes. J. Nanobiotechnol. 2022; 20 (1): 86. DOI: https://doi.org/10.1186/s12951-022-01283-2 PMID: 35180876; PMCID: PMC8857816.

13. Ning F., Liu H., Lash G.E. The role of decidual macrophages during normal and pathological pregnancy. Am. J. Reprod. Immunol. 2016; 75: 298–309. DOI: https://doi.org/10.1111/aji.12477 PMID: 26750089.

14. Bogdanova I.M., Nizyaeva N.V., Artemyeva K.A., Boltovskaya M.N., Kondashevskaya M.V. Involvement of mast cells in physiological and pregnancy complicated by preeclampsia. Immunologiya. 2022; 43 (6): 736–45. https://doi.org/10.33029/0206-4952-2021-42-6-737–745. (in Russian)

15. Rajakumar A., Kane M.A., Yu J., Jones J.W., Qu H., Badell M., Taylor R.N., Sidell N. Alternatively activated macrophages are the primary retinoic acid-producing cells in human decidua. Reprod. Sci. 2020; 27 (1): 334–41. DOI: https://doi.org/10.1007/s43032-019-00030-7 PMID: 32046391; PMCID: PMC7539807.

16. Kowalska W. Expression of CD163 and HLA-DR molecules on the monocytes in chronic lymphocytic leukemia patients. Folia Histochem. Cytobiol. 2020; 58 (1): 17–24. DOI: https://doi.org/10.5603/FHC.a2020.0002 PMID: 32176313.

17. Yamashita M., Utsumi Y., Nagashima H., Nitanai H., Yamauchi K. CD163 expression profles in classical monocytes as biomarkers to discriminate idiopathic pulmonary fibrosis from idiopathic nonspecifc interstitial pneumonia. Sci. Rep. 2021; 11: 12135. DOI: https://doi.org/10.1038/s41598-021-91407-9 PMID: 34108546; PMCID: PMC8190107.

18. Babania O., Mohammadi S., Yaghoubi E., Sohrabi A., Seyedhosseini F. S., Abdolahi N., Yazdani Y. The expansion of CD14+ CD163+ subpopulation of monocytes and myeloid cells-associated cytokine imbalance; candidate diagnostic biomarkers for celiac disease (CD). J. Clin. Lab. Anal. 2021; 35 (10): 23984. DOI: https://doi.org/10.1002/jcla.23984 PMID: 34449925; PMCID: PMC8529138.

19. Krijgsman D., De Vries N.L., Andersen M.N., Skovbo A., Tollenaar R.A.E.M., Møller H.J., Hokland M., Kuppen P.J.K. CD163 as a biomarker in colorectal cancer: the expression on circulating monocytes and tumor-associated macrophages, and the soluble form in the blood. Int. J. Mol. Sci. 2020; 21 (16): 5925. DOI: https://doi.org/10.3390/ijms21165925 PMID: 32824692; PMCID: PMC7460610.

20. Greco R., Demartini C., Zanaboni A.M, Tumelero E., Persico A., Candeloro E., Morotti A., Amantea D., Tassorelli C. CD163 as a potential biomarker of monocyte activation in ischemic stroke patients. Int. J. Mol. Sci. 2021; 22: 6712. DOI: https://doi.org/10.3390/ijms22136712 PMID: 34201498; PMCID: PMC8268853.

21. Nunes P.R., Romão-Veiga M., Peraçoli J.C., Araujo Costa R.A., de Oliveira L.G., Borges V.T.M., Peraçoli M. T. Downregulation of CD163 in monocytes and its soluble form in the plasma is associated with a pro-inflammatory profile in pregnant women with preeclampsia. Immunol. Res. 2019; 67 (2–3): 194–201. DOI: https://doi.org/10.1007/s12026-019-09078-8 PMID: 31240481.

22. Zelinka-Khobzey M.M., Tarasenko K.V., Mamontova T.V., Shlykova O.A. Characteristics of CD68+ and CD163+ expression in placenta of women with preeclampsia and obesity. Wiad. Lek. 2021; 74 (9 cz 1): 2152–8. DOI: https://doi.org/10.36740/WLek202109122 PMID: 34725292.

23. Sinyakova A.A, Shipitsyna E.V., Budilovskaya O.V., Bolotskikh V.M., Savicheva A.M. Anamnestic and microbiological predictors of miscarriage. Zhurnal akusherstva i zhenskikh bolezney. 2019; 68 (2): 59–70. DOI: https://doi.org/10.17816/JOWD68259-70 (in Russian)

24. Yao Y., Xu X., Jin L. Macrophage polarization in physiological and pathological pregnancy. Front. Immunol. 2019; 10: 792. DOI: https://doi.org/10.3389/fimmu.2019.00792 PMID: 31037072; PMCID: PMC6476302.

25. Bogdanova I.M., Artem’eva K.A., Boltovskaya M.N. Development and function of regulatory B cells and its role in pregnancy support. Immunologiya. 2021; 42 (4): 415–25. DOI: https://doi.org/10.33029/0206-4952-2021-42-4-415-425 (in Russian)

26. Wang L.X., Zhang S.X., Wu H.J., Rong X.L., Guo J. M2b macrophage polarization and its roles in diseases. J. Leukoc. Biol. 2019; 106 (2): 345–58. DOI: https://doi.org/10.1002/JLB.3RU1018-378RR PMID: 30576000; PMCID: PMC7379745.

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