Dual effect of amino acid compositions upon antibacterial activity of human neutrophilic granulocytes

Abstract

Introduction. It is known that amino acid compositions for parenteral nutrition exhibit an immunomodulatory effect on T and B lymphocytes, phagocytes.

The aim of the study was to evaluate the effect of amino acid compositions on the antibacterial functions of human peripheral blood neutrophilic granulocytes (neutrophils) under various experimental conditions.

Material and methods. Neutrophils were preincubated with amino acid compositions Amin or Vamin 14, then phorbol-myristate-acetate (PMA) was added and the respiratory burst of neutrophils was evaluated by flow cytometry. In another model neutrophils were incubated with bacteria (St. aureus) in a ratio of 1 : 10 or 10 : 1, washed, incubated with amino acid compositions, washed, lysed and sown on meat-peptone agar to account for colonies formed by surviving bacteria.

Results. Amin and Vamin 14 had a weak immunostimulating effect on the respiratory burst of neutrophils activated by PMA. The addition of amino acid compositions to neutrophils that phagocytized bacteria at a neutrophil/bacteria ratio of 1 : 10 led to an increase in the number of colonies formed by the surviving bacteria. With a neutrophil/bacteria ratio of 10 : 1, the studied amino acid compositions enhanced the bactericidal activity of neutrophils, causing a decrease in the number of surviving bacteria forming colonies. Direct addition of amino acid compositions enhanced colony formation by bacteria.

Conclusion. The amino acid compositions Amin and Vamin 14 enhance the growth of bacteria, including phagocytized by neutrophils at a cell/bacteria ratio of 1 : 10, but stimulate the bactericidal activity of neutrophils phagocytized St. aureus at a cell/bacteria ratio of 10 : 1, as well as in the respiratory burst induction test in neutrophils activated by PMA.

Keywords:amino acid composition Amin; amino acid composition Vamin 14; human blood neutrophils; respiratory burst; bactericidal activity

For citation: Potapnev M.P., Andreyev S.V., Goncharova N.V., Viatkina O.I., Berdina E.L., Gapanovich V.N. Dual effect of amino acid compositions upon antibacterial activity of human neutrophilic granulocytes. Immunologiya. 2023; 44 (1): 28–37. DOI: https://doi.org/10.33029/0206-4952-2023-44-1-28-37 (in Russian)

Funding. The study was supported by the grant the Ministry of Health the Republic of Belarus No. 20210507.

Conflict of interests. Authors declare no conflict of interests.

Authors’ contributions. The concept and design of the study – Potapnev M.P., Andreyev S.V.; data collection and processing – Andreyev S.V., Viatkina O.I.; flow cytometry – Goncharova N.V., work with bacterial cultures – Viatkina O.I., statistical data processing – Andreyev S.V., Goncharova N.V., text writing – Andreyev S.V., Berdina E.L., Goncharova N.V., article editing – Gapanovich V.N., Potapnev M.P.

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