Influence of the recombinant vaccine against pseudomonas infection on humoral immune response and protective activity in mice
AbstractPseudomonas infection is a serious socio-economic problem of public health, as the pathogen has a high level of resistance to antibiotics, and the clinical manifestations of the disease are characterized by diversity and, often, severe course. The aim – study of antibody response in mice vaccinated with candidate vaccine against Pseudomonas aeruginosa, characterization of the spectrum of subisotypes of the produced immunoglobulins.
Material and methods. Mice were injected intraperitoneally at 2-week intervals with a recombinant vaccine against P. aeruginosa (25 µg of OprF and 50 µg of a-Tox (toxoid), sorbed on 225 µg of aluminum hydroxide gel). 3 weeks after the last immunization of mice, antibody levels were determined by ELISA and the protective activity of the vaccine upon infection with a lethal dose of P. aeruginosa.
Results. Twice immunization of mice with a recombinant vaccine against P. aeruginosa induced an increase (p < 0.01) in mice blood of IgM and IgG titers, as well as IgG1, IgG2a, IgG2b and IgG3 isotypes to OprF and a-Tox compared to control (non-vaccinated mice). At the same time, the vaccine protected mice from a lethal infecting dose of P. aeruginosa in 80% of cases.
Conclusion. It can be assumed that the action of the vaccine based on recombinant OprF protein and P. aeruginosa toxoid is associated not only with the induction of antibodies, but also with the activation of the cellular mechanisms of the immune response.
Keywords:toxoid; OprF; a-Tox Pseudomonas aeruginosa; immunoglobuline isotypes
Received 27.05.2019 Accepted 16.06.2019
For citation: Influence of the recombinant vaccine against pseudomonas infection on humoral immune response and protective activity in mice. Immunologiya. 2019; 40 (4): doi: 10.24411/0206-4952-2019-14003.
Acknowledgments. The study had no sponsorship.
Conflict of interest. The authors declare no conflict of interest.
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