The comparative analysis of the phenotypic profile of peripheral blood B lymphocytes and decidua in uncomplicated pregnancy and preeclampsia

• Natalia Yu. Sotnikova
• Anna V. Kudryashova
• Irina A. Panova
• Anna I. Malyshkina
• Zaira S. Khizrieva
• Kirill D. Rukavishnikov

Abstract

Introduction. B-lymphocytes can play an important role in the regulation of immune processes during pregnancy, which may be significantly different at systemic level and in placenta due to multiple factors of cell microenvironment and pregnancy complications.

The aim of the study was to establish the character of differentiation of B-lymhocytes loca­lized in decidua comparing to that in peripheral blood in normal pregnancy and preeclampsia

Material and methods. The material for examination were samples of peripheral blood andleucocyte infiltrate of decidua (LID). 67 women and 21 placentas were examined in compa­rison group without hypertensive disorders during pregnancy and 100 women and 31 placentas in the group of women with preeclampsia (PE). The study was done by flow cytometry method. The level of naïve cells (CD27^–IgD⁺), memory cells (CD27⁺IgD^±), «nonswitched» (CD27⁺IgD⁺), «switched» (CD27⁺IgD^–) memory and plasma cells (CD20^–CD38⁺) in CD19⁺ В-lymphocyte population was assessed. The level of CD5⁺В1а cells, IL-10⁺ Breg (CD20⁺IL-10⁺), antigen presenting HLA-DR⁺ cells was assessed in CD20⁺ B-lymphocyte population.

Results. In the LID of placenta comparing with peripheral blood: 1) in the comparison group and in the PE group there was a lower level of Breg and «switched» memory B-cells; 2) in the comparison group the level of B1a cells and «nonswitched» memory B-cells was higher, but the content of naive B-cells was lower; 3) in the PE group there was a smaller fraction of the total population of memory B cells. In contrast to the comparison group, in LID with PE the level of CD20⁺-B-lymphocytes, B1a cells and naive B-cells was higher, and the amount of memory B-cells and «non-switched» memory B-cells was significantly lower. The duration of manifestation and the severity of PE were not associated with the nature of differentiation of decidual B-lymphocytes.

Conclusion. The character of B-lymphocyte differentiation is different at systemic level and in placenta in normal pregnancy and preeclampsia.

Keywords: B-lymphocytes; cell differentiation; pregnancy; preeclampsia

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